Coding

Part:BBa_K1074009

Designed by: Changlong Zhao   Group: iGEM13_USTC_CHINA   (2013-09-16)


SdpA,SdpB,SpbC

Killing factor of Bacillus subtilis ,SpbC Induces the lysis of other B.subtilis cells that have not entered the sporulation pathway, providing a source of nutrients to support sporulation. Its maturation is dependent on SdpA and SdpB. Also functions as a ligand, being able to bind to SdpI thereby triggering a signal transduction cascade that protects the cell against the toxic effects of its own SdpC. We use this killing factor to build a kill switch for Bacillus subtilis.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 572
    Illegal PstI site found at 1999
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 572
    Illegal PstI site found at 1999
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 572
    Illegal PstI site found at 1999
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 572
    Illegal PstI site found at 1999
    Illegal AgeI site found at 759
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 746


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Categories
Parameters
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